NAD+ 500MG

£39.99

Purity: ≥ 99%

£39.99

NAD+ 500MG

Name: NAD+ 500MG
Brand: Pure Grade Labs
Price: 39.99 GBP
Availability: OutOfStock

£39.99

Purity: ≥ 99%

£39.99

CELLULAR ENERGY - How your cells produce energy

LONGEVITY GENES - Activating your body's anti-ageing pathways

DNA REPAIR - Fixing daily cellular damage

AGEING & DECLINE - Why NAD+ drops as you get older

NAD+ (β-Nicotinamide Adenine Dinucleotide) is a coenzyme present in every living cell, formed from two nucleotides joined by a pyrophosphate bridge - one carrying an adenine base, the other a nicotinamide ring derived from vitamin B3. It exists in two interconvertible forms: NAD+ (oxidised) accepts electrons from metabolic substrates and is reduced to NADH, which then feeds those electrons into the mitochondrial electron transport chain to drive ATP production. Without NAD+ c

Beyond its classical redox role, NAD+ is consumed as a substrate by three families of enzymes that have made it a central target in ageing research: sirtuins (SIRT1-7), which deacetylate proteins to regulate metabolism, DNA repair, and stress resistance; PARPs (poly-ADP-ribose polymerases), which use NAD+ to flag DNA damage for repair; and CD38, an immune-cell ectoenzyme whose activity rises with age. Tissue NAD+ levels decline measurably with age in animal models and several human tissues, and this decline correlates with mitochondrial dysfunction, sarcopenia, and metabolic disease. Restoring NAD+ - either directly or via precursors like NR (nicotinamide riboside) and NMN (nicotinamide mononucleotide) - is one of the most actively investigated longevity interventions in current clinical research.

Redox Function: NAD+/NADH is the dominant electron-carrier pair in cellular metabolism. Glycolysis, the TCA cycle, and beta-oxidation all depend on NAD+ as the electron acceptor. The NAD+/NADH ratio sets intracellular redox state and gates metabolic flux. Reduced NAD+ availability impairs ATP synthesis at the mitochondrial level.

Signalling Function: Sirtuins, PARPs, and CD38 consume NAD+ as a co-substrate, not a co-factor - meaning they degrade it. Sirtuin-driven deacetylation of PGC-1α, FOXO, and p53 underlies many of the metabolic and stress-resistance phenotypes attributed to NAD+ restoration. CD38 upregulation with age is a major driver of NAD+ decline.

Key Structural Features: Dinucleotide: nicotinamide mononucleotide (NMN) + adenosine monophosphate (AMP) joined by pyrophosphate bridge | Two forms: NAD+ (oxidised) / NADH (reduced) | Substrate for sirtuins, PARPs, and CD38 | Highest concentrations in mitochondria (40-70% of cellular pool)

CAS Number: 53-84-9

Form: Free Acid

Molecular Weight: 663.43 g/mol

Appearance: White Powder

Direct NAD+ supplementation in humans is logistically more demanding than precursor supplementation, so the strongest published evidence base concerns oral precursors (NR, NMN) that elevate the NAD+ metabolome. The studies below are drawn from peer-reviewed publications. Pure Grade Labs does not interpret these findings as evidence of efficacy in humans outside of their stated research contexts.

Nature Communications: Chronic NR supplementation elevates NAD+ in healthy middle-aged and older adults and reduces blood pressure (n=30)

Cell Reports: NR augments the aged human skeletal muscle NAD+ metabolome and induces an anti-inflammatory transcriptomic signature (n=12)

NPJ Aging: Chronic NMN supplementation elevates whole-blood NAD+ and improves muscle function in older men (n=42)

References

https://pubmed.ncbi.nlm.nih.gov/34238308/

https://pubmed.ncbi.nlm.nih.gov/29599478/

https://pubmed.ncbi.nlm.nih.gov/31412242/

https://pmc.ncbi.nlm.nih.gov/articles/PMC4112140/

https://pubmed.ncbi.nlm.nih.gov/32694684/

https://pubmed.ncbi.nlm.nih.gov/31278280/

Every batch of NAD+ supplied by Pure Grade Labs is accompanied by a Certificate of Analysis (COA) from an independent third-party analytical laboratory. The COA confirms identity, purity by HPLC, and absence of contaminants. Batch-specific documentation is linked below.

NAD+ is supplied as a lyophilised (freeze-dried) hygroscopic white powder. It is light-sensitive and degrades rapidly in solution at ambient temperature. Correct handling is essential to preserve coenzyme integrity. Follow the specifications below.

Temperature: -20°C Long-term storage. For short-term use (up to 4 weeks), 2-8°C is acceptable.

Light Sensitivity: Light-Sensitive. Store in original opaque vial. Avoid direct UV or prolonged exposure to light.

Shelf Life: 24 Months. Lyophilized, sealed, stored at -20°C. Batch expiry printed on vial label.

Allow the sealed vial to reach room temperature before opening to minimise condensation. NAD+ is hygroscopic and will absorb atmospheric moisture rapidly once exposed.
Use bacteriostatic water (BAC water) or sterile water for injection as the reconstitution solvent. Add solvent slowly down the inside wall of the vial - do not inject directly onto the lyophilised cake.
Gently swirl until fully dissolved. NAD+ is highly water-soluble and should dissolve quickly. Do not vortex or shake vigorously.
Inspect the solution: it should be clear and colourless to very pale yellow. Discard if cloudy, deeply discoloured, or contains particulate matter.
Once reconstituted, store at 2-8°C protected from light. Use within 5-7 days. Solution-phase NAD+ is significantly less stable than lyophilised material.

NAD+ supplied by Pure Grade Labs is intended exclusively for in vitro research and laboratory use. NAD+ is not approved by the FDA, MHRA, EMA, or any other regulatory body as a drug or medicine for the treatment, prevention, cure, or mitigation of any human disease or condition.

This product is not a drug, medicine, or supplement and must not be used as such outside of a licensed clinical or research context.
Pure Grade Labs makes no claims regarding therapeutic or clinical efficacy in humans for any use of this research compound.
Direct exogenous NAD+ administration in humans has limited long-term safety data. Most controlled human evidence concerns oral NAD+ precursors (NR, NMN), which are distinct molecules.
Reported adverse signals from intravenous NAD+ infusion include flushing, nausea, chest tightness, and transient blood pressure changes during administration.
Subcutaneous administration of NAD+ is not an approved or established route in any clinical context.
Purchasers are solely responsible for compliance with all applicable laws and regulations in their jurisdiction.

All product information, research summaries, and study citations presented on this page are provided for informational context only and do not constitute medical advice, endorsement of any treatment, or recommendation for human use.